Using artificial sweeteners may set the stage for diabetes in some people by hampering the way their bodies handle sugar, according to a preliminary study carried out mostly on mice.

The authors said they are not recommending any changes to how people use artificial sweeteners based on their study, which included some human experiments. The researchers and outside experts said more study is needed, while industry groups called the research limited and said other evidence shows sweeteners are safe and useful for weight control.

The study, by researchers in Israel, was released on Wednesday by the journal Nature.

The work suggests the sweeteners change the composition of normal, beneficial bacteria in the gut. That appears to hamper how well the body handles sugar in the diet, which in turn can result in higher blood sugar levels. This impairment, called glucose intolerance, can eventually lead to diabetes.

Some experts who didn't take part in the work urged caution in interpreting the results.

James Hill, an obesity expert at the University of Colorado, called the work good science.

Still, overall, "I do not think there is enough data yet to lead to a definitive conclusion about artificial sweeteners and the body's handling of sugar," he wrote in an e-mail. "I certainly do not think there is sufficient evidence to conclude that they are harmful."

But Yanina Pepino of Washington University in St Louis said the results make a convincing case that sweeteners hamper the body's handling of sugar by altering gut bacteria. This adds to her belief that sweeteners and sugar should be used in moderation, especially by children, she said.

"It's really providing strong data suggesting we need to do more research," she added.

Test in mice

Researchers began by testing three widely used sweeteners - saccharin, sold for example as Sweet'N Low; sucralose, sold as Splenda; and aspartame, sold for example as NutraSweet - in 20 mice. Some animals got one of these substances in their water, and others got sugar water or just water. After 11 weeks, researchers gave all the mice a dose of sugar and monitored the response in their blood sugar levels.

The mice that initially got sugar showed about the same response as those that got plain water. But mice that got any of the sweeteners showed markedly higher blood sugar levels, indicating impairment in handling of the sugar dose. Further mouse experiments linked that outcome to an effect on gut bacteria.

The researchers turned to 381 non-diabetic volunteers who filled out a questionnaire that estimated their consumption of artificial sweeteners. Forty participants who had the most showed evidence of higher blood sugar than 236 nonusers.